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. 2017 Dec 2;93(1):107–118. doi: 10.1111/tpj.13761

Figure 1.

Figure 1

Characterization of the grik1‐2 grik2‐1 mutant line.

(a) Schematic diagram of grik1 T‐DNA insertion lines.

(b) Reverse transcriptase‐polymerase chain reaction (RT‐PCR) of full‐length GRIK1, GRIK2 and tubulin8 in the wild‐type Col‐0, grik1‐2, grik2‐1 and grik1‐2 grik2‐1.

(c) Phenotype of the wild‐type and grik1‐2 grik2‐1 under short‐day condition for 4 weeks.

(d) Northern blotting with the middle region of GRIK1 as probe in the wild‐type, grik1‐1 and grik1‐2.

(e) cDNA sequence of the 5′‐end region of grik1‐2 and potential ORFs. The longest ORF corresponded to an N‐terminal truncated GRIK1 protein starting at Met‐156 of the wild‐type protein.

(f) Amount and phosphorylation status of SnRK1s in the wild‐type and grik1‐2 grik2‐1.

Western blot with anti‐pAMPK antibody (upper panel) and anti‐SnRK1.1 antibody (middle panel). Coomassie staining was used to show protein amounts (lower panel). A greater amount of proteins from grik1‐2 grik2‐1 (150%) was also loaded to compare the phosphorylation ratio in equivalent amounts of SnRK1.1.