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. 2017 Dec 2;93(1):107–118. doi: 10.1111/tpj.13761

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© 2017 The Authors. The Plant Journal published by John Wiley & Sons Ltd and Society for Experimental Biology.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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SOS2‐GRIK1 interaction visualized by bimolecular fluorescence complementation (BiFC).

Panels from (a) to (f) show several images of reconstituted BiFC by GRIK1‐SOS2 interaction (left: fluorescence images; right: overlay with transmitted light). Arrows indicate the presence of a transvacuolar strand in (c) and (d), and nuclei in (e) and (f). Panels (g) and (h) show a negative control with GRIK1 in pSPYCE(M) and the empty vector pSPYNE(R)173. Panels (i) and (j) show co‐transformation with SOS2 in pSPYNE(R)173 and the empty vector pSPYCE(M). Scale bar: 10 μm.