. 2018 Feb 16;6(1):coy008. doi: 10.1093/conphys/coy008

Table 1:

Biochemical validation for cortisol enzyme immunoassay for all species used for validation and field research

Habitat	Species	Study	Location	Parallelism^a	Percent recovery^b
Terrestrial	Green treefrog	Validation; field	Captive; wild	0.996	y = 0.891x + 3.389; R² = 0.997; P < 0.001
Terrestrial	American toad	Validation; field	Captive; wild	0.988	y = 0.985x + 0.207; R² = 0.990; P < 0.001
Semi-aquatic	Northern leopard frog	Validation; field	Captive; wild	0.993	y = 0.887x + 0.113; R² = 0.999; P < 0.001
Semi-aquatic	Cricket frog	Validation	Wild	0.993	y = 1.180x + 0.798; R² = 0.995; P < 0.001
Aquatic	Axolotl	Validation	Captive	0.997	y = 0.833x + 6.262; R² = 0.993; P < 0.001
	Red-spotted newt	Validation	Captive	0.963	y = 0.887x + 0.113; R² = 0.999; P < 0.001
	Mudpuppy	Validation	Captive	0.992	y = 0.985x + 0.827; R² = 0.999; P < 0.001
	American Bullfrog	Field	Wild	0.991	y = 1.160x + 2.823; R² = 0.999; P < 0.001
	Green frog	Field	Wild	0.996	y = 0.891x + 3.389; R² = 0.997; P < 0.001
	Western chorus frog	Field	Wild	0.985	y = 0.834x + 0.903; R² = 0.999; P < 0.001
	Spring peeper	Field	Wild	0.995	y = 1.176x + 0.422; R² = 0.999; P < 0.001
	Tiger salamander	Field	Wild	0.993	y = 1.150x + 0.485; R² = 0.998; P < 0.001
	Rough-skinned newt	Validation	Captive	0.996	y = 0.834x + 0.903; R² = 0.999; P < 0.001
	Blue-spotted salamander	Field	Wild	0.997	y = 1.020x + 1.500; R² = 0.999; P < 0.001
	Hellbender	Validation	Captive	0.751	y = 1.005x + 0.641; R² = 0.999; P < 0.001

^aParallelism compares the relationship between cortisol standards and serially diluted swab samples (2× concentrated to 1:16) separately for all species calculated using Pearson’s Product Moment correlation.

^bPercent recovery calculated as a best fit line using a linear regression of observed over expected values when known amounts of cortisol standard is added to a sample.