Figure 11.

The effect of the alkylation on the primer extension reaction. (A) Gel image of primer extension reaction. The alkylation was initially carried out with (CTG)₂₀ ODN10 or (CAG)₂₀ ODN11 (5 μM) and VDAT–acridine conjugate 2 (100 μM) in MES buffer (50 mM, pH 7.0) containing NaCl (100 mM) and 2% DMSO at 37°C for 6 h (lane 2 or 6) or 24 h (lane 3 or 7). For TAT–acridine conjugate 1, the mixture was incubated for 24 h (lane 4 or 8). The ODNs were then annealed with the primer ODN9. The primer extension reactions were performed with the template ODN10 or ODN11 (0.5 μM) and primer ODN9 (0.17 μM), Klenow Fragment (exo−) (0.1 U/μl) and 0.2 mM dNTP in buffer (pH 7.9) at 37°C for 10 min. (B) The ratio of full length production.