Figure 10.

Triplex-directed targeting of DNA nanostructures: Triplex sequence addressability of various DNA architectures. (A) Targeting of an individual sequence within a hexagonal array generated from three-way branched oligonucleotides. The complex contains an appropriately positioned FRET pair (F1 and F2) that allows the association and dissociation of the TFO to be monitored by pH change. Adapted from (90) with permission. (B) Triplex-directed scaffolding of a double-crossover tile and array with a streptavidin proteins. Adapted from (91) with permission. (C) Targeting of an individual sequence positioned within a DNA origami frame. Binding of the TFO results in the association of the two duplexes running along the centre of the frame into an X-shaped structure that is visualised by AFM. Adapted from (93) with permission. (D) Triplex- directed scaffolding of a tensegrity triangle crystal with a cyanine dye. Adapted from (94) with permission.