Figure 2.

Identification and isolation of three stable complexes of Thermotoga maritima Tsa proteins. (A) Native agarose gel electrophoresis analysis of individual T. maritima Tsa proteins (lanes 1–4) and various equimolar mixtures (lanes 5–15). (B) Size-exclusion chromatographic (SEC) profiles of individual TsaB (gray dashed line) and TsaD (dotted line) proteins, of the TsaB₂D₂ complex (black dashed line) formed from an equimolar mixture of TsaB and TsaD, of the TsaB₂D₂E₁ complex (gray solid line) formed from an equimolar mixture of TsaB, TsaD and TsaE, and of the TsaB₂D₂E₂ complex (black solid line) formed by adding excess TsaE to an equimolar mixture of TsaB and TsaD. All SEC profiles were collected by monitoring absorbance at 280 nm. Inset: SDS-PAGE analysis of SEC fractions corresponding to the major SEC peaks for the protein mixtures.