Table 1. Parameters calculated from SAXS data.

	TsaB2D2	TsaB2D2E2
Structural parameters
Rg (Å) from Guinier plot	44.20 ± 0.06	48.22 ± 0.07
Rg (Å) from P(r)	44.86 ± 0.08	47.84 ± 0.1
Dmax (Å)	148.9	150
Porod volume estimate, Vp (Å3)	250,783	289,003
Molecular mass (kDa)
From DATMOW	129.22	158.85
From DAMMIN models	121.35	141.35
From theoretical protein sequence	128	166
Ab initio modeling parameters
DAMAVER NSD (averaged over 10 models)a	0.532 ± 0.142	0.69 ± 0.04

^aNSD: normalized spatial discrepancy.