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. 2017 Nov 23;74(3):672–681. doi: 10.1002/ps.4761

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© 2017 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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PS-4761-FIG-0004-b — PCR detection of the 519‐bp MgMFS1 promoter insert in Zymoseptoria tritici strains using primers MFF1 and MFR1. Samples of EasyLadder I (Bioline, London, UK) in lanes 1 and 16, products of strains Orleans 26 (lane 2), Lyon 35 (3), R15‐46 (4), Orleans 8 (5), Lyon 26 (6), Orleans 6 (7), Lyon 24 (8), Orleans 12 (9), Orleans 11 (10), Lyon 14 (11), Orleans 40 (12), Lyon 31 (13), Lyon 16 (14), NT321.17 (15), IPO323 (17) and Flu‐6 (18). No amplification for Lyon 24, Lyon 31 and water control (not shown). Largest product (1009 bp) in lane 14 and 15, Lyon 16 and NT321.17, respectively, indicates the presence of 519 bp promoter insert; no insert present in smaller 490 bp product.