Table 2.
Sensitivity profiles of laboratory‐generated Sdh mutants and filed isolates of Z. tritici against six SDHIs
| Isolate | Mutation in Sdh subunit | Amino acid substitution | Resistance factor (RF)a | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Originb | Boscalid | Bixafen | Fluxapyroxad | Penthiopyrad | Fluopyram | Isofetamid | |||
| M38 | B | D129T | lab | 32.3 | 118.1 | 211.2 | 206.4 | 30.8 | 13.7 |
| Iso − 13 | B | P220T | lab | 4.1 | 2.0 | 2.4 | 1.1 | 9.6 | 40.7 |
| M152 | B | S221P | lab | 1.9 | 2.5 | 0.9 | 3.1 | 1.2 | 4.6 |
| M6 | B | R265P | lab | 15.2 | 10.0 | 13.3 | 6.2 | 3.7 | 2.9 |
| M46 | B | R265P | lab | 15.9 | 6.5 | 11.9 | 10.8 | 3.2 | 3.6 |
| 18 − 11 | B | H267Y | lab | > 64 | 13.0 | 34.6 | 26.5 | 0.6 | 0.5 |
| M36 | B | H267Y | lab | > 64 | 22.0 | 48.0 | 40.3 | 1.0 | 0.3 |
| 15‐8 | B | H267L | lab | > 64 | > 363 | > 284 | > 213 | 45.3 | 110.2 |
| V5 − 1 | B | T268I | lab | 5.7 | 9.5 | 15.7 | 13.0 | 2.4 | 4.4 |
| V5 − 12 | B | T268I | lab | 7.5 | 12.2 | 17.2 | 9.8 | 2.9 | 2.8 |
| M62 | B | I269V | lab | 7.4 | 7.4 | 12.4 | 6.6 | 11.2 | 7.3 |
| M96 | B | I269V | lab | 7.7 | 9.3 | 13.4 | 7.8 | 12.4 | 10.1 |
| 20 − 13 | C | T79I | lab | > 64 | > 363 | > 284 | > 213 | 11.0 | 7.2 |
| 16 − 12 | C | S83G | lab | > 64 | > 363 | 207.7 | > 213 | > 51 | 28.1 |
| Flu‐6 | C | A84V | lab | 1.7 | 2.3 | 0.7 | 2.2 | 18.1 | > 174 |
| Iso‐30 | C | L85P | lab | 13.5 | 39.1 | 39.8 | 87.9 | 15.1 | 33.1 |
| M142 | C | N86 K | lab | > 64 | > 363 | > 284 | > 213 | 38.2 | 24.6 |
| 12 − 17 | C | H152R | lab | > 64 | > 363 | > 284 | > 213 | 28.3 | 24.0 |
| V9C‐23 | C | I161S | field | 2.1 | 6.8 | 5.4 | 1.5 | 1.4 | 1.6 |
| V6A‐9 | C | T79 N, V128 M | field | 10.1 | 12.4 | 16.3 | 16.4 | 3.6 | 4.9 |
| M112 | D | D129E | lab | > 64 | 14.9 | 20.8 | 5.9 | 2.5 | 3.6 |
| IPO323 (EC50; mg L−1) | none | field | 0.16 | 0.03 | 0.04 | 0.05 | 0.20 | 0.06 | |
a
Ratio between EC50 value of each isolate and of IPO323. Owing to dose–response curve‐fitting and/or solubility, 10 mg L−1 was used as the cut‐off value for EC50 determination. Each value is based on the means of two individual EC50 values.
b
IPO323‐derived laboratory mutant (lab) or UK field isolates collected in Norfolk (V6A‐9) and Wiltshire (V9C‐23) in 2015.