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. 2017 Nov 20;46(3):1139–1156. doi: 10.1093/nar/gkx1160

Figure 2.

Figure 2.

DRN deficient strains display elevated Levels of UPR marker transcripts and protein: (A) Histogram showing the abundance of transcripts encoding ER chaperones BiP and PDI in a Wild type (yBD-117), cbc1-Δ (yBD-131), rrp6-Δ (yBD-129) strains in presence tunicamycin at 30°C. Three independent cDNA preparations (biological replicates, n = 3) were used to determine the levels of these mRNAs. Normalized value of individual mRNA from normal samples was set to 1. The statistical significance of difference as reflected in the ranges of P-values estimated from Student's two-tailed t-tests for a given pair of test strains for each message are presented with following symbols, ∗P < 0.05, ∗∗P < 0.005 and ∗∗∗P < 0.001, NS, not significant. (B) Relative steady state level of Bip/KAR2 protein in wild type (yBD-117), cbc1-Δ (yBD-131), rrp6-Δ (yBD-129) strains in the presence of 1 μg/ml tunicamycin (+Tm) at 30°C. α-Tubulin (lower panel) was used as the loading control. Cells of indicated strains were grown in the absence and in the presence of tunicamycin, harvested and total protein extract were prepared as described in Materials and Methods. 50 μg of total cellular protein was loaded in each lane, resolved in 12% SDS-PAGE and probed with anti-Bip and anti-tubulin antibodies to detect these proteins as described in Materials and Methods.