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. 2017 Nov 20;46(3):1139–1156. doi: 10.1093/nar/gkx1160

Figure 5.

Figure 5.

Relative levels of transcript segments corresponding to HAC1 Exon 1 and 3′-BE in absence of ER-stress. (A) Histogram showing the relative abundance of transcript segments corresponding to HAC1 exon 1 and HAC1 3′-BE element in normal, cbc1-Δ, and rrp6-Δ strains in the absence of tunicamycin as determined from three independent experiments (biological replicates) and are presented as means ± SE (n = 3 for each strain). Normalized value of individual amplicon from normal strain sample was set to 1. (B) Northern blot revealing the levels of transcript segments corresponding to HAC1 exon 1 and HAC1 3′-BE level in a wild type (yBD-117), cbc1-Δ (yBD-131), rrp6-Δ (yBD-129) strains in the absence of ER stress. The northern blot was hybridized with a DNA oligonucleotide encompassing either an HAC1-Exon1 sequence or HAC1-3′-BE element sequence respectively. (C) Composite graph revealing the relative abundance of the CBC1 message, HAC1 Exon 1 and 3′-BE element in a wild type yeast strain in the absence of ER-stress harboring an engineered allele of CBC1 gene expressed under pGAL1-GAL10 promoter at different times post-induction with 2% galactose. (D) Relative growth of wild type (yBD-117), hac1-Δ (yBD-118), hac1-Δ pRS316 (yBD-404) hac1-Δ pHAC1 (yBD-405), hac1-Δ pHAC1-NRE (yBD-406) and hac1-Δ pHAC1-BE (yBD-407) strains in YPD solid growth media in absence (–Tm) and in presence of 1 μg/ml tunicamycin (+Tm).