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. 2017 Nov 20;46(3):1139–1156. doi: 10.1093/nar/gkx1160

Table 1. Half Life values of HAC1 pre-mRNA in different strains in presence and absence of ER stress.

Pertinent genotype Half-lives (in minutes)
Without ER stress With ER stress
WT 4.8 9.8
cbc1-Δ 25.25 28.63
rrp6-Δ 20.05 22.75
*

The half-lives were determined from three independent mRNA Decay Experiment (biological replicates) in which relevant yeast strains were grown till mid-log phase followed by the induction of ER Stress, shutting off the transcription with 1,10-phenanthroline, measuring the specific mRNA levels by Real-time quantitative PCR analyses and plotting the data as percent mRNA remaining as a function of time as described in Materials and Methods.