Table 1. Half Life values of HAC1 pre-mRNA in different strains in presence and absence of ER stress.
Pertinent genotype | Half-lives (in minutes) | |
---|---|---|
Without ER stress | With ER stress | |
WT | 4.8 | 9.8 |
cbc1-Δ | 25.25 | 28.63 |
rrp6-Δ | 20.05 | 22.75 |
The half-lives were determined from three independent mRNA Decay Experiment (biological replicates) in which relevant yeast strains were grown till mid-log phase followed by the induction of ER Stress, shutting off the transcription with 1,10-phenanthroline, measuring the specific mRNA levels by Real-time quantitative PCR analyses and plotting the data as percent mRNA remaining as a function of time as described in Materials and Methods.