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. 2017 Nov 27;66(3):592–605. doi: 10.1002/glia.23267

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© 2017 The Authors GLIA Published by Wiley Periodicals, Inc.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Injection of Ad‐shGLUT2 into the 3V inhibits GLUT2 without altering GK and GLUT1. (a) Experimental protocol. (b‐d) Analysis of GLUT2, GK, and GLUT1 mRNA expression relative to cyclophilin in hypothalamic samples from rats transduced with Ad‐shβgal or Ad‐shGLUT2 by qRT‐PCR. (e) Western blot analysis of hypothalamic protein extracts isolated from rats transduced with Ad‐shβgal (lane 1) or Ad‐shGLUT2 (lane 2) for 48 hr. (f‐h) Densitometric analysis of each protein relative to β‐actin evaluated in Ad‐shGLUT2‐injected rats, as a percentage of levels observed in Ad‐shβgal‐EGFP‐injected rats. Results are representative of four independent experiments performed in triplicate. Data are expressed as mean ±SD. * p < .05, ** p < .01 t‐test [Color figure can be viewed at wileyonlinelibrary.com]