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. 2017 Dec 12;46(3):1256–1265. doi: 10.1093/nar/gkx1244

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© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Survival of UvrA⁻ cells exposed to UV. (A) Spot plates of decreasing cell titers exposed to 5 J/m² UV (254 nm) show improved survival with ectopic UvrB or UvrC. Lane 1 UvrA⁻ cells, lane 2 UvrA⁻ cells complemented with UvrA-eGFP, lane 3 UvrA⁻ cells complemented with UvrB-eGFP, lane 4 UvrA⁻ cells complemented with UvrC-eGFP. (B) Quantification of spot plates by colony counting. Survival of UvrA⁻ cells complemented with eGFP-tagged NER proteins versus UV dose shows a significant improvement in survival at low UV doses (5–10 J/m²) for UvrC-complemented cells. Cell survival is shown in logarithm units and error bars indicate standard deviation.