Figure 1.

(a) Diagram of the experimental procedure used in a Tn‐seq approach. A Tn5 insertion library (transposons depicted as green boxes) is constructed by transformation and used in growth assays in different conditions, in order to assess differential growth of each insertion mutant. After harvesting the cells, the extracted genomic DNA is processed by DNA shearing, A‐tailing, and ligation of adapters (blue boxes), followed by an enrichment using PCR. This enriched library is subsequently sequenced by NGS and the results of the number of readings in the different conditions are compared in order to identify the differential abundance of insertions. (b) The method used in this study for generating the Tn5 library generating a combination of miniTn7‐Gm and miniTn5‐Km. The efficiency of the method to generate random insertion libraries was assessed by counting colonies of a sample