Table 1.
Plasmids and strains used in this study
| Genotype | Source | |
|---|---|---|
| Plasmid | ||
| pBK‐miniTn7‐gfp2 | pUC19‐based delivery plasmid for miniTn7‐gfp2. GmR, CmR, ApR, mobq | Koch et al. (2001) |
| pTNS2 | R6K replicon‐based helper plasmid, providing the Tn7 transposition functions in trans. ApR Mob+ | Choi et al. (2005) |
| pUT‐Km | R6K replication origin‐based suicide delivery plasmid for miniTn5‐Km. ApR KmR | Herrero et al. (1990) |
| pRK600 | ColE1 oriV; RP4tra+ RP4oriT; CmR; helper in triparental matings | Kessler, de Lorenzo, and Timmis (1992) |
| pEMG | oriV(R6K), lacZα fragment with I‐SceI sites; KmR | Martínez‐García and de Lorenzo (2011) |
| pEMG‐vacJ | oriV(R6K), lacZα fragment with I‐SceI sites, with 500 bp of gene vacJ; KmR | This study |
| pEMG‐tyrB | oriV(R6K), lacZα fragment with I‐SceI sites, with 500 bp of gene tyrB; KmR | This study |
| pEMG‐fleN | oriV(R6K), lacZα fragment with I‐SceI sites, with 500 bp of gene fleN; KmR | This study |
| Strains | ||
| P. putida KT2440 | Mt‐2 hsdR1 (r‐ m+) | Bagdasarian et al. (1981) |
| P. putida KT2440 Δfcs | P. putida KT2440 derivative with gene fcs deleted | Calero et al. (2016) |
| P. putida KT2440::miniTn7‐Gm:GFP | P. putida KT2440 with Gentamycin resistance gene in a miniTn7 | This study |
| P. putida KT2440 Δfcs::miniTn7‐Gm:GFP | P. putida KT2440 Δfcs with Gentamycin resistance gene in a miniTn7 | This study |
| P. putida KT2440 ttg2A‐ | P. putida KT2440 derivative with a miniTn5‐Km insertion gene ttg2A | Duque et al. (2007) |
| P. putida KT2440 ttg2B‐ | P. putida KT2440 derivative with a miniTn5‐Km insertion gene ttg2B | Duque et al. (2007) |
| P. putida KT2440 ccmC‐ | P. putida KT2440 derivative with a miniTn5‐Km insertion gene ccmC | Duque et al. (2007) |
| P. putida KT2440 ccmF‐ | P. putida KT2440 derivative with a miniTn5‐Km insertion gene ccmF | Duque et al. (2007) |
| P. putida KT2440 vacJ‐ | P. putida KT2440 derivative with an insertion in gene vacJ | This study Duque et al. (2007) |
| P. putida KT2440 tyrB‐ | P. putida KT2440 derivative with an insertion in gene tyrB | This study Duque et al. (2007) |
| P. putida KT2440 fleN‐ | P. putida KT2440 derivative with an insertion in gene fleN | This study Duque et al. (2007) |
| E. coli K‐12 MG1655 | F‐ hsdS gal | |
| E. coli DH5α | ϕ80dlacZΔM15 Δ(lacZYA‐argF)U169 recA1 endA1 hsdR17 (rk ‐ mk+) supE44 thi‐1 gyrA relA1 | Hanahan (1985) |