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Visualization of the situation after a 20 μL loop has been filled for 1 min at 10 μL/min. The 1D eluent species are depicted as dots. The magnitude of the flow velocities is represented by the colour. Analyte molecules to the right of the vertical line in the middle would be lost if the loop volume and sample volume were equal. Visualization created using computational fluid dynamics by Dr. Suhas Nawada (University of Amsterdam)
