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. 2017 Nov 29;46(3):1167–1179. doi: 10.1093/nar/gkx1180

Figure 6.

Figure 6.

Sim3 is the effector for the GRANT motif under the regulation of cis–trans isomerases in safeguarding chromosome segregation. (A) Suppression of chromosome missegregation defects in mutants of GRANT motif and isomerases—P13A, P15A, P13AP15A and Δani1Δani2 by ectopic expression of sim3+His using pRep41. N > 150, two tailed t-test, *P < 0.05. Bar plot indicates the mean of three biological replicates. Vec, empty vector. WT, wild type (B) Physical interaction between Sim3 and wild type (WT) or Cnp1-4PA (4PA) NTDs in the presence or absence of Ani1 and/or Ani2 isomerases (Δani1, Δani2, Δani1Δani2). Extracts were prepared from WT and single and double mutants of Δani1 and Δani2 cells expressing Sim3-FLAG; and Cbh1-HA, Bead, beads only control; Pept PD, peptide pull down; Lad, molecular weight ladder. Cbh1-HA, positive and loading control. Representative image of experimental duplicates. Relative enrichment intensity measured against WT peptide pull down in WT cells, adjusted for loading with Cbh1-HA.