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Stains-all (A) and Western blotting (B) analysis of purified GPI-anchored glycoconjugates from Leishmania major (positive control) and Leptomonas pyrrhocoris parasites. Glycoconjugates (20 µg per lane) from late-log stage cultures were probed with the WIC 79.3 monoclonal antibody (1:1000), specific for β1,3Gal residues of LPG. Protein ladder sizes on the left are in kDa.
