Figure 4.

Caulobacter ParB binds to parS and spreads to flanking DNA in a heterologous E. coli host. A cassette composed of individual parS (red line) site and an apramycin resistance marker aac(3)IV was inserted at the yybD locus on an E. coli chromosome. T18-ParB (WT) (black) or T18-ParB (G101S) (blue) were expressed from an IPTG-inducible promoter, and their distribution on the E. coli chromosome were determined by α-T18 ChIP-seq. ChIP-seq signals were reported as the number of reads at every nucleotide along the genome (RPBPM value). A cassette composed of a scrambled parS site 3 and an apramycin resistance marker was also inserted at the yybD locus and serves as a negative control.