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. 2017 Nov 23;46(3):1196–1209. doi: 10.1093/nar/gkx1192

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© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — The position of an ectopic parS on the chromosome is critical for the fitness of Caulobacter. Micrograph of parB::cfp-parB Caulobacter cells (A) without an extra ectopic parS³⁺⁴, (B) with an extra ectopic parS³⁺⁴ at +200 kb, (C) at +1000 kb or (D) at +1800 kb. Cell length of an exponentially-growing cells were quantified and presented as histograms. Vertical dotted red lines indicate the mean cell length. The number of CFP-ParB foci (green) per cell was also quantified and plotted as histograms. Note that we could not observe foci corresponding to an extra ectopic parS³⁺⁴ perhaps due to the limited numbers of ParB bound to this shorter cluster. Most observable foci are likely due to the original parS^1–7 cluster that reside ∼8 kb near ori.