Figure 4.

Down-modulation of MALAT1 inhibits anti-apoptotic genes. T98G cells were transfected with 100 nM scL-siMAL for 24 h, followed by 1000 μM TMZ for 72 h. The controls included treatments with either scL, scL-siCTRL, or free siMAL in combination with TMZ as well as with TMZ alone. Expression of MALAT1 (A), MGMT (B), and MRP1 (C) were assessed by RT-PCR performed in triplicate. (D) Expression of anti-apoptotic proteins was determined using antibody array performed in duplicate. NS, not significant. *P < 0.05, **P < 0.001.