Figure 2.

DNA sequence flanking the binding sites modulate Egr-1 binding. Three Egr-1 binding sites are integrated, separately, into a C1 and C2 DNA context (Supplementary Figure S3) and subjected to multiple unzipping cycles in the presence of 500 nM Egr-1. (A) Mean breaking force and (B) binding probability are presented for sites -3, -2, -1 and the consensus motif. The data is analyzed as in Figure 1. The number of experiments for each case is shown in Supplementary Table S1, and the P-values in Supplementary Table S5.