Figure 5.

Unzipping Egr-1 from the ZF3 direction requires less force (A) Schematic representation of forward and reverse unzipping. The Lhb promoter or the consensus motif in the C1 DNA context, are subjected to multiple cycles of forward (F) or reverse (R) unzipping in the presence of 500 nM Egr-1. The data for binding probability (B) and breaking force (C) are analyzed and presented as in Figure 1. The number of experiments for each case is shown in Supplementary Table S1, and the P-values in Supplementary Table S8.