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. 2017 Mar 9;19(4):303–310. doi: 10.1001/jamafacial.2016.2162

Figure 4. Effects of Exercise on Flap Phosphorylated Protein Kinase B (p-Akt) Activation and Vascular Endothelial Growth Factor A (VEGFA) Expression.

Figure 4.

These graphs illustrate the relative fold increase over the baseline level of activated (Ser-473 or Thr-308) p-Akt and VEGFA as detected by multistrip Western blotting in pooled protein samples of the proximal, middle, or distal segments obtained at postoperative days 2, 5, and 9 or from excised defect during surgery (postoperative day 0) in the resting animal group (RAG) and exercise animal group (EAG). A, p-Akt signal intensity values detected were first normalized to p-Akt, growth factor receptor–bound protein 2 (Grb2), and β-actin mean signal intensity values, averaged and then plotted as fold induction (FI) in reference to basal p-Akt levels. B, Densitometric VEGFA signal intensity values were normalized to Grb2 and β-actin signal intensity mean values and then plotted as FI in reference to baseline VEGF levels. Data are expressed as mean (SD) (68% CI) from a technical triplicate.