Table 1. Histomorphometric Variables in the Diet and Photoaging Experiment.
| Variable | Group, Mean (SD) | Group, Mean (SD) | Group, Mean (SD) | Comparison of All 6 Groups | Comparison of Control Groups, P Value | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| C | CR | P Value | CrC | CrR | P Value | OC | OR | P Value | ANOVA F Ratio | P Value | C vs CrC | C vs OC | |
| Wrinkles, Rz value | 103.6 (27.7) | 84.3 (25.1) | NS | 63.4 (15.1) | 79.9 (13.1) | NS | 101.1 (28.9) | 76.1 (25.2) | .03 | 3.48 | <.01 | .001 | NS |
| Epidermal thickness | 9.4 (1.2) | 17.7 (6.7) | <.01 | 21.0 (7.7) | 28.2 (11.8) | <.03 | 7.9 (0.0) | 18.5 (2.8) | <.002 | 7.72 | <.001 | <.001 | NS |
| Epidermal PCNA index | 15.7 (10.0) | 41.1 (2.6) | <.001 | 57.3 (5.0) | 62.4 (5.9) | NS | 17.8 (3.1) | 49.1 (2.6) | <.001 | 3.61 | <.01 | <.01 | NS |
| Collagen I immunostaining, % | 130.7 (10.3) | 160.5 (6.5) | <.01 | 165.9 (6.4) | 159.6 (7.1) | NS | 127.0 (14.2) | 138.6 (11.4) | <.02 | 23 | <.001 | <.01 | NS |
| Elastic fibers, % | 42.3 (8.3) | 51.9 (11.9) | <.02 | 67.5 (8.1) | 60.8 (11.4) | NS | 44.8 (13.5) | 63.5 (15.8) | <.002 | 6.41 | <.001 | <.001 | NS |
| Fibroblasts, No. | 7.3 (4.6) | 25.7 (4.4) | <.001 | 31.0 (2.9) | 21.7 (2.9) | <.001 | 9.1 (1.9) | 15.0 (4.8) | <.003 | 49.7 | <.001 | <.01 | NS |
| Mast cells, No. | 13.8 (1.5) | 19.9 (2.7) | <.01 | 14.5 (3.5) | 10.6 (1.1) | <.01 | 10.3 (2.5) | 17.8 (3.0) | <.01 | 34.52 | <.001 | NS | <.01 |
| Dermal cellularity | 18.5 (3.1) | 21.6 (2.8) | NS | 20.8 (4.0) | 15.4 (1.9) | <.05 | 14.6 (3.2) | 22.5 (4.5) | <.001 | 6.71 | <.001 | NS | <.05 |
| Adipose layer ratio | 0.60 (0.07) | 0.55 (0.06) | NS | 0.63 (0.01) | 0.63 (0.03) | NS | 0.70 (0.06) | 0.64 (0.05) | NS | 3.74 | <.01 | NS | <.05 |
Abbreviations: ANOVA, analysis of variance; C, intact control with regular diet and no UV-B exposure; CR, intact control with UV-B exposure; CrC, calorie-restricted diet; CrR, calorie-restricted diet with UV-B exposure; NS, not significant; OC, obesity diet; OR, obesity diet with UV-B exposure; PCNA, proliferating cell nuclear antigen; Rz, surface roughness.