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. 2018 Jan 4;46(3):1345–1361. doi: 10.1093/nar/gkx1284

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© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 9. — The U-rich motif is required for CspA auto-regulation in vivo. mRNA and protein levels of CspA^3xFLAG expressed from pCspA^3xFLAG (A), pCspA^3xFLAG-M5U (B) and pCspA^3xFLAG-M5UC (C) in the WT and ΔcspA strains. Total RNA and protein extraction were performed as indicated above. Northern blots were then developed using a ³²P-labeled anti-FLAG oligo probe and autoradiography. Processed cspA^3xFLAG mRNA is indicated with an asterisk. Ethidium bromide staining of 16S RNA is shown as loading control (LC). Western blots were developed using peroxidase conjugated anti-FLAG antibodies and a bioluminescence kit. Coomassie stained gel portions are shown as loading controls (LC).