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Extended phosphorylated life time of CnYpd1. The histidine kinase and receiver domains from the heterologous donor ScSln1 (ScSln1-HKR1) were used as a phospho-donor for CnYpd1. Phosphorylated ScSln1-HKR1 was removed from the reaction and aliquots of CnYpd1 were taken at specified time points. Three replicates were performed.
