Figure 5.

CnYpd1 ΔN70 dephosphorylation. CnYpd1 ΔN70 was incubated with phospho∼GST-ScSln1-HKR1. GST-ScSln1-HKR1 was removed from the reaction. Aliquots were removed and mixed with stop buffer to quench the reaction. (A) All data points for phosphorylated half-life experiments as performed in quadruplicate for CnYpd1 ΔN70. Each colored line on the plot represents a separate experiment. (B) Representative phosphorimage that shows dephosphorylation of CnYpd1 ΔN70 over time. A phosphorylated half-life of 3.4 ± 0.63 h (N = 4) was calculated.