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. Author manuscript; available in PMC: 2018 Feb 16.
Published in final edited form as: J Immunol. 2010 Mar 15;184(8):4247–4257. doi: 10.4049/jimmunol.0902914

Figure 6. Depletion of CD11chiMHCIImed cells disrupts FRC reassembly and vascular barrier function.

Figure 6

Mice were injected with BMDCs on day 0, received intraperitoneal DT or CRM on day 6, and were analyzed on day 7. (A–B) Desmin+ FRC organization around and near T zone HEV at (A) 160x and (B) 240x. Arrows highlight examples of perivascular FRCs that are more loosely associated or protruding out from the vessel wall. (C) gp38+ FRC organization around HEV. Arrows highlight examples of perivascular FRCs that are protruding out from the vessel wall. For (A–C), photos are representative of at least 5 mice per condition. (D) Percentage of T zone HEV with disrupted FRC organization. At least 19 fields over 5 lymph nodes were assessed for each condition in CD11c-DTR mice. **=p<.001. (E) Relative vessel permeability as assessed by Evans blue extravasation. Evans blue has red fluorescence and vascular permeability is reflected by the relative degree of red fluorescence (47). Photos are representative of at least 3 mice per condition.