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Bacterial strains and plasmids used in this study
| Strains/plasmids | Description | References |
|---|---|---|
| Strains | ||
| BL21(DE3)pLysS | F-ompT hsdSB (rB- mB-) dcm galλ (DE3) pLysS Cmr | Promega |
| JOL1682 | E. coli BL21(DE3) pLysS expressing LfliC via pET28a (+) system | This study |
| Plasmids | ||
| pET28(+) | IPTG-inducible, T7 expression vector, C-terminal 6×His tag, KanR | Novagen, USA |
| pET28(+)-LfliC | pET28a derivative containing LfliC | This study |
