Table 2.
Typical examples of nVGDS for bone tissue repair and regeneration
| Chemical vector | Scaffold/matrices | Wound type | Animal/cell | DNA/RNA | References |
|---|---|---|---|---|---|
| PEI | Collagen scaffold | Calvarial defects | Fisher 344 rats/human BMSCs | pDNA-PDGF-B | [109] |
| PEI coated with PEG | Poly-(ε-caprolactone) scaffold coated with poly-(d,l-lactide) | None | C2C12 cells | pDNA-BMP-2 | [110] |
| PEI/FuGENE6 | Calcium phosphate cement scaffold | Calvarial defects | Mice | pDNA-caALK6 and pDNA-Runx2 | [111] |
| PEI | PEG hydrogels | None | hMSCs | siNoggin or miRNA-20a | [112] |
| Cationized gelatin microspheres (CGMS) | Oligo (poly(ethylene glycol) fumarate) (OPF) hydrogel | Cranial defects | Rat | pDNA-BMP-2 | [114] |
| TAPP/gelatin microparticles (GMPs) | PPF scaffold | Cranial defect | Rat | pDNA-BMP-2 | [115] |
| Chitosan-disulfide-conjugated low molecular weight-PEI | None | None | MG-63 osteoblast cells and stem cells | pDNA-BMP-2 | [116] |
| (K)16GRGDSPC | PLGA-[ASP-PEG]n matrices | Segmental femoraldefects | Rabbit/rabbit-derived BMSCs | pDNA-TGF-b1 | [118] |
| FuGENE6™ | None | None | Rat/osteoblasts | pDNA-TGF-b1 | [119] |
| Lipofectamine 2000 | None | None | BMSC | AntimiR-138 (oligonucleotide) | [120] |
| Lipid (DOTAP-2-dioleoyl-sn-glycero-3-phosphatidylethanolamine/DOTAP-cholesterol) | None | None | Osteoblastic cell lines (MG63 and MC3T3-E1) | pDNA-β-gal | [121] |
| Calcium phosphate (CAP) | PLL film | None | Human osteoblasts | ShRNA(mouseSpp1andBglap-rs1) | [123] |
| Nanohydroxyapatite | Colagen-nHA scaffold | Cranial defect | Rat/MSCs | pDNA-VEGF and pDNA-BMP2 | [126] |
| PEI-LA | Gelatin/collagen scaffolds | Subcutaneous implantation model | Rat | pDNA-bFGF and pDNA-BMP-2 | [127] |
| Lipofectamine 2000 (coprecipitated within apatite) | PLGA films | None | C3H10T1/2 cell | pDNA-β-gal | [128] |