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. 2017 Jan 30;30(3):227–237. doi: 10.1177/0394632016689571

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© The Author(s) 2017

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 3.0 License (http://www.creativecommons.org/licenses/by-nc/3.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 2. — IL-6 induced calcification of the HUASMCs. (a) The control group was treated without rhIL-6 for six days and showed a negative result of calcium stain by alizarin red S staining. (b) The experimental group was treated with rhIL-6 (50 ng/mL) for the same time period and the extracellular matrix was positively stained with orange. The two arrows indicated the sand-like and the nodular calcium stain in the extracellular matrix, respectively. Both panels were magnified 200 times. (c) Cells were treated with different concentrations of rhIL-6 for different durations as indicated. Calcium concentrations were elevated in a time- and dose-dependent manner. *P<0.05 vs. control of the same time point or vs. the sample of the earlier time point within the same group. **P<0.05 vs. control and sample of the other group at the same time point.