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. Author manuscript; available in PMC: 2018 Feb 16.
Published in final edited form as: J Comp Neurol. 2017 Aug 21;525(17):3666–3682. doi: 10.1002/cne.24295

FIGURE 2.

FIGURE 2

Validation of the ERβ-EGFP mouse model for studying PVN ERβ expression. Representative confocal photomicrographs comparing the distribution of ERβ-EGFP in a female mouse (similar pattern is observed in males) (a) with ERβ-ir (c), using the Z8P antibody in the middle-caudal PVN. Enlarged images (b, d, f) shows a higher power view demonstrating the distribution of ERβ-EGFP (green) and ERβ-ir cells (magenta). And their colocalization (e, f). For all high power insets (dotted line box) the scale bar = 10 μm. Scale bars for all other images = 50 μm. 3V, Third ventricle [Color figure can be viewed at wileyonlinelibrary.com]