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. Author manuscript; available in PMC: 2018 Feb 16.
Published in final edited form as: J Comp Neurol. 2017 Aug 21;525(17):3666–3682. doi: 10.1002/cne.24295

FIGURE 6.

FIGURE 6

ERα/ERβ-EGFP colocalized cells are present in low levels in the male and female mouse PVN. Photomicrographs of a female ERβ-EGFP mouse showing ERα immunoreactivity (magenta) in the rostral (a–d), middle (e–h), and caudal (i–l) parts of the PVN of ERβ-EGFP animals (n = 3–4/sex). Bar graphs (panel m) represent the mean percentage colocalization of ERα in ERβ-EGFP neurons of male and female mice. % colocalization = number of colocalized ERα/total number of ERβ neurons in selected region. Data are expressed as mean percentage ± SEM. For all high-power images (dotted line box) (d, h, l) the scale bar = 10 μm. Scale bars for all other images = 50 μm. 3V, Third ventricle. Arrowheads show examples of dual labeled cells [Color figure can be viewed at wileyonli-nelibrary.com]