TABLE 1.
Validation of antibodies used
| Primary antibodies | ||||
|---|---|---|---|---|
| Target | Antigen | Source | Working dilution | Reference |
| Arginine vasopressin | Full length AVP peptide | Dr. James Koenig (Univ. MD) Rabbit polyclonal | 1:2,500 | Kasting and Martin (1983), Kasting et al. (1985) |
| Estrogen receptor α | Rat ERα C-term | Upstate #06–935 Rabbit polyclonal | 1:5,000 | Suzuki and Handa (2005), Cao et al. (2014), RRID:AB_310305 |
| Estrogen receptor β | ERβ mC-term (468–485) | Zymed Z8P (no longer available) rabbit polyclonal | 1:1,000 | Shughrue and Merchenthaler (2001), Suzuki and Handa (2005), McClellan et al. (2010) |
| GFP | GFP-1020 | Aves: Chicken polyclonal | 1:1,000 | Cao et al. (2014), RRID:AB_10000240 |
| Oxytocin | Full length OT peptide | Peninsula: T4084 rabbit polyclonal | 1:5,000 | Sutton et al. (2014) |
| Tyrosine hydroxylase | Synthetic rat TH aa: 32–46 | Abcam: ab6211: rabbit polyclonal | 1:1,000 | Wasserman, Wang, Rashid, Josselyn, and Yeomans (2013). RRID:AB_2240393 |
| Secondary antibodies | ||||
| Antibody | Source | Catalog No. | Working dilution | |
| Goat anti-Chicken IgY (H&L)DyLight® 594 | ThermoFisher | SA5–10072 | 1:500 RRID:AB_2556652 |
|
| Goat anti-Rabbit IgG (H&L) AlexaFluor® 594 | Life Technologies | A-11037 | 1:500 RRID:AB_2534095 |
|
| Goat biotinylated anti-rabbit IgG (H+L) | Vector Laboratories | BA-1000 | 1:500 RRID: AB_2313606 |
|
| Streptavidin DyLight® 594 | Vector Laboratories | SA5594 | 1:500 RRID:AB_2336418 |
|
| TSA Fluorescein | Perkin Elmer | NEL744001KT | 1:50 | |
| TSA Cy3 | Perkin Elmer | NEL744001KT | 1:50 | |
| ABC Kit Biotinylated/SA-HRP | Vector | PK-6104 | RRID:AB_2336823 | |