Figure 2. Hepatic Transcript Amplitude Is Increased in Mice Lacking Nocturnin.

Cycling hepatic transcripts display amplitude increases in mice lacking NOC (Noct^−/−, KO) during the dark phase.

(A) Relative amplitude (AMP_Rel) was determined for cycling transcripts (q < 0.05) by taking the ARS algorithm amplitude value and dividing it by the average expression (reads per kilobase of transcript per million mapped reads [RPKM]) value for a given transcript across all time points (Table S2). Transcripts were grouped by peak phase and grouped into day (ZT0–ZT12) and night (ZT12–ZT24) categories. The number of transcripts is indicated on each bar. Averages for transcripts were graphed according to all amplitudes (left) or those with AMP_Rel > 0.15.

(B) AMP_Rel was plotted as in (A), but with transcripts cycling with a q < 0.15 threshold.

(C and D) Cycling transcripts from (B) with AMP_Rel > 0.15 were subdivided into 3 hr peak phase windows. Transcripts were grouped into 3 hr bins ± 1.5 (e.g., Zeitgeber time (ZT) 3 transcripts had a peak phase ranging from ZT1.5 to ZT4.5) and presented according to genotype with WT (C) or KO (D).

Unless otherwise noted, bars represent the mean ± SEM and significance was determined using a Student’s t test.