Figure 3. Inhibition of KCa3.1 activity changes Ca²⁺ signaling in BMMs.

A) Representative snapshots obtained from time-lapse videos showing Ca²⁺ signals during acute stimulation with RANKL [100 ng/mL] in Fluo-4-loaded BMM. B) Representative fluorescence traces of Ca²⁺ signals during RANKL stimulation of a control (left) and a 3 µM TRAM-34 pretreated cell (right). C) Analysis of Ca²⁺ responses in respective experiments; unpaired Student’s t-test and Mann-Whitney-U test were performed when applicable; overall response: control: N=13, TRAM-34: N=9; duration: control: N=37, TRAM-34: N=24; amplitude: control: N=34, TRAM-34: N=24; shown are means ± SEM, ****P<0.0001.