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. 2018 Feb 7;38(6):1588–1599. doi: 10.1523/JNEUROSCI.1925-17.2017

Figure 4.

Figure 4.

Effects of orexin on the LH/PFVgat → Orx input. A, Orexin-A (300 nm) produces no changes on photo-evoked IPSC amplitude. Gray represents 30 photo-evoked IPSCs. Black represents average photo-evoked IPSCs. B, Time course of photo-evoked amplitude before, during, and after orexin application (mean photo-evoked IPSCs; n = 7). C, D, Orexin has no significant effect on photo-evoked IPSC latency (C, n = 7; one-way ANOVA, F = 0.223, p = 0.803) or photo-evoked IPSC probability (D, n = 7; one one-way ANOVA, F = 2.882, p = 0.089). E, F, In the same neurons in which orexin has no effects on the photo-evoked IPSCs, orexin increases the sIPSC frequency. Cumulative distribution plots of sIPSC interevent intervals compiled from 7 orexin neurons (E; 200 ms bins; two-way ANOVA, F = 2.276, p < 0.001; Bonferroni's multiple-comparisons post hoc test, *p < 0.05 comparing control and OxA). Mean values of sIPSC frequency (F; n = 7; one-way ANOVA, F = 4.113, p = 0.0393; **p < 0.01, Fisher's LSD post hoc test). IPSCs were evoked by 10 ms light pulses and were recorded at Vh = 0 mV using the Cs-methane-sulfonate-based pipette solution.