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. Author manuscript; available in PMC: 2018 Feb 16.
Published in final edited form as: Eur J Pharmacol. 2016 Apr 13;785:24–35. doi: 10.1016/j.ejphar.2016.04.024

Fig. 8. TLR2/1 dimerization determined by TR-FRET in monocytes.

Fig. 8

TR-FRET assay was used to detect dimerization of TLR2 with TLR1 using anti-TLR2. Antibodies labeled with europium cryptate as the donor fluorophore and anti-TLR1. Antibodies labeled with d2 as the acceptor fluorophore: an assay to detect the dimerization of native receptors in the context of intact cell membrane, whereas, X-ray crystallography or immunoprecipitation assays to detect dimerization uses truncated or epitope tagged recombinant receptor protein, respectively. (Snodgrass et al., 2013).