Table 1.
Antibodies used in the study.
| Protein | MW | Antibody | Dilution | Reference | Supplier |
|---|---|---|---|---|---|
| Primary antibodies: | |||||
| DYRK1A | 90 KDa | Rabbit anti-DYRK1A | 1:2000 | 2771 | Cell Signaling |
| pAkt | 70 KDa | Rabbit anti-P(S473)-Akt | 1:2000 | 9271 | Cell Signaling |
| Akt | 70 KDa | Rabbit anti-Akt | 1:2000 | 9272 | Cell Signaling |
| pGSK-3β | 46 KDa | Mouse anti-P(Tyr216)-GSK-3β | 1:2000 | 612313 | BD Biosciences |
| pGSK-3β | 46 KDa | Rabbit anti-P(Ser9)-GSK-3β | 1:2000 | 9336 | Cell Signaling |
| GSK-3β | 46 KDa | Rabbit anti-GSK-3β | 1:2000 | sc-9166 | Santa Cruz |
| Bax | 20 KDa | Rabbit anti-Bax | 1:2000 | 2772 | Cell Signaling |
| Bim | 23 KDa | Rabbit anti-Bim | 1:1000 | B7929 | Sigma-Aldrich |
| Bcl-XL | 28 KDa | Rabbit anti-Bcl-XL | 1:2000 | PA1-14066 | Pierce |
| Bcl-W | 18 KDa | Rabbit anti-Bcl-W | 1:1000 | MA5-15076 | Pierce |
| βTub | 49 KDa | Mouse monoclonal anti-β-Tubulin | 1:5000 | T4026 | Sigma-Aldrich |
| Secondary antibodies: | |||||
| IgG | Goat anti-rabbit IgG peroxidase conjugate | 1:2000 | A6154 | Sigma-Aldrich | |
| IgG | Goat anti-mouse IgG peroxidase conjugate | 1:2000/1:5000 | A4416 | Sigma-Aldrich | |
β-tub: β-tubulin; Akt, protein kinase B; DYRK1A: dual specificity, tyrosine phosphorylation regulated kinase 1A; GSF-3β: glycogen synthase kinase-3β: IgG: immunoglobulin G; MW: molecular weight.