Figure 4.

HEATR1 knockdown induces ribosomal stress A, B. U2OS cells were transfected with indicated siRNAs, fixed and immunostained with fibrillarin (FBL), nucleophosmin (NPM), nucleolin (NCL) and p53 antibodies 72 h after transfection. Nuclei were visualized with DAPI staining. Bar, 10 µm. C. U2OS cells were transfected with indicated siRNA, lysed and immunoblotted with indicated antibodies 72 h after transfection. D. U2OS cells were transfected with control or HEATR1 siRNAs. Cell lysates were immunoprecipitated 72 h after transfection with control (IgG) or RPL5 antibodies and immunoblotted with indicated antibodies.