Examples of laminar patterns of labeled projection neurons directed to A25. A, Wide column through area 28 shows darkly labeled neurons in the deep layers (arrowheads, Case BU). B, Higher-magnification inset from layer V shows labeled neurons (arrowheads). C, Higher-magnification inset from B shows labeled axon terminations in layer V (e.g., arrowheads). D, Grayscale epifluorescence shows wide column through area 14 with brightly labeled neurons in layers III and V–VI (e.g., arrowheads, Case BP). E, Wide column through area 12 shows darkly labeled neurons in the superficial layers (arrowheads, Case BU). F, Higher-magnification inset of layer III shows labeled neuron (arrowhead). G, Higher-magnification inset of labeled axon terminations in layer III (e.g., arrowhead). Dotted lines indicate laminar boundaries. ld, Lamina dissecans; WM, white matter.