Table S1.
Draize’s scale of weighted scores for grading the severity of ocular lesions
| Assessment | Score |
|---|---|
| Cornea | |
| A. Opacity – degree of density (most dense area taken for reading) | |
| No ulceration or opacity | 0 |
| Scattered or diffuse area; details of iris clearly visible | 1 |
| Easily discernible translucent areas; details of iris slightly obscured | 2 |
| Opalescent areas; no details of iris visible, size of pupil barely discernible | 3 |
| Opaque; iris invisible | 4 |
| B. Area of cornea involved | |
| None | 0 |
| One-quarter (or less) but not zero | 1 |
| Greater than one-quarter, less than one-half | 2 |
| Greater than one-half, less than three-quarters | 3 |
| Greater than three-quarters, up to whole area | 4 |
| Score = A × B ×5 (range, 0 to 80) | |
| Iris | |
| A. Values | |
| Normal | 0 |
| Folds above normal, congestion, swelling and/or circumcorneal injection; iris still reacting to light (sluggish reaction is positive) | 1 |
| No reaction to light, hemorrhage and/or gross destruction | 2 |
| Score = A ×5 (range, 0 to 10) | |
| Conjunctivae | |
| A. Redness of palpebral conjunctivae | |
| Normal | 0 |
| Vessels definitely injected above normal | 1 |
| More diffuse, deeper crimson red; individual vessels not easily discernible | 2 |
| Diffuse beefy red | 3 |
| B. Chemosis | |
| Normal | 0 |
| Any swelling above normal (includes nictitating membrane) | 1 |
| Obvious swelling with partial eversion of the lid | 2 |
| Swelling with lids about half closed | 3 |
| Swelling with lids about half closed to completely closed | 4 |
| C. Discharge | |
| Normal | 0 |
| Any amount different from normal | 1 |
| Discharge with moistening of the lids and hair just adjacent to the lids | 2 |
| Discharge with moistening of the lids and considerable area around the eye | 3 |
| Score = (A + B + C) ×2 (range, 0 to 20) | |
| Total score=Sum of all scores obtained for the cornea, iris and conjunctivae |