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. 2017 Nov 6;37(8):982–992. doi: 10.1038/onc.2017.394

Figure 1.

Figure 1

NUAK1 is required for calcium-dependent activation of AMPK. (a) Whole-cell extracts from U2OS cells treated with the indicated concentrations of HTH-01-015 for 1, 6 or 16 h and probed with the indicated antibodies. (b) Lysates from U2OS cells pre-treated with HTH-01-015 for 1 h prior to stimulation with 3 μm A23187 (10 min), 100 μm A769662 (1 h) or 10 mm Phenformin (1 h) and blotted for phospho- and total ACC. Densitometry shows p-ACC levels in the image shown. (c) Lysates from equal numbers of U2OS and HeLa cells were probed for LKB1. Images are from the same gel and immunoblot, but rearranged to omit extraneous data. (d) Lysates from HeLa cells pre-treated with HTH-01-015 for 1 h prior to stimulation with 3 μm A23187 (10 min), 100 μm A769662 (1 h) or 10 mm Phenformin (1 h) and blotted for phospho- and total ACC. Densitometry shows p-ACC levels in the image shown. All images are representative of at least three independent experiments.