Table I.
CHO HCP impurities with attractive interactions to mAbs A–D, Fc D and Fab D at 150 mM NaCl, pH 7.4.
| Protein ID | mAb A | mAb B | mAb C | mAb D | Fab D | Fc D | Location | MW (kDa) | pI |
|---|---|---|---|---|---|---|---|---|---|
| Neural cell adhesion molecule | − | + | + | + | − | − | Cell membrane | 17.9 | 4.7 |
| Renin receptor | − | + | − | + | + | − | Cell membrane | 33.9 | 5.2 |
| Lipoprotein lipase | + | + | + | − | − | + | Cell membrane, secreted | 50.5 | 7.9 |
| Chondroitin sulfate protoglycan 4 | + | − | + | + | + | − | Cell membrane, cell projection, membrane | 153.8 | 6.1 |
| Alpha-enolase | − | + | − | − | − | − | Cytoplasm, cell membrane | 15.5 | 5 |
| Galectin-3-binding protein | + | − | − | + | − | + | Membrane | 63.8 | 5.1 |
| G-protein coupled receptor 56 | + | − | + | + | − | + | Membrane | 77.4 | 9.1 |
| V-type proton ATPase subunit S1 | − | + | − | − | − | − | Membrane, vacuole | 28.1 | 6.5 |
| Nidogen-1 | + | + | + | + | − | + | Basement membrane, extracellular matrix | 30.1 | 8.1 |
| ATP synthase subunit beta, mitochondrial | + | + | − | − | − | + | Mitochondrion inner membrane | 56.3 | 5.2 |
| Vimentin | + | + | − | − | − | − | Cytoplasm | 53.7 | 5.1 |
| Heat shock protein | + | + | − | − | − | − | Cytoplasm | 32.6 | 5.7 |
| Actin | + | + | − | + | − | + | Cytoplasm | 41.5 | 5.2 |
| Peroxirodoxin 1 | − | − | − | − | − | + | Cytoplasm, melanosome | 22.3 | 8.2 |
| SPARC | + | + | + | + | + | − | Secreted | 28.2 | 4.9 |
| Clusterin | + | + | + | + | + | − | Secreted | 51.8 | 6.1 |
| Complement C1r-a sub-component | + | − | − | − | − | − | Extracellular | 80.1 | 6.1 |
| Metalloproteinase inhibitor 1 | + | − | − | − | − | − | Secreted | 22.4 | 8.8 |
| Insulin | + | + | + | + | − | − | Secreted | 28.1 | 5.5 |
| Cathepsin D | − | + | − | − | − | − | Lysosome, melanosome | 44.9 | 6.7 |
| Sulfated glycoprotein 1 | + | − | − | − | − | − | Lysosome | 27.4 | 5.4 |
| Lysosomal protective protein | − | − | − | − | − | + | Lysosome | 54 | 6.1 |