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. Author manuscript; available in PMC: 2018 Feb 16.

Published in final edited form as: Bioorg Med Chem. 2016 Jun 2;24(17):3918–3931. doi: 10.1016/j.bmc.2016.05.071

Dark ( ) and phototoxicity ( ) of selenorhodamine thioamide 5 (a) toward HUT-78 cells with 0.0 and 1.0 J cm⁻² of laser light and varying concentrations of 5 and (b) with 5 × 10⁻⁸ M 5 ( ) and varying light dose (0.0, 0.5, 1.0, and 2.0 J cm⁻²) delivered at 630 ± 2 nm and a fluence rate of ~3.2 mW cm⁻². (c) Dark ( ) and phototoxicity ( ) of selenorhodamine amide 6 toward HUT-78 cells with 0.0 and 1.0 J cm⁻² of laser light and varying concentrations of 6 and (d) with 5 × 10⁻⁸ M 6 ( ) and varying light dose (0.0, 0.5, 1.0, and 2.0 J cm⁻²) delivered at 630 ± 2 nm and a fluence rate of ∼ 3.2 mW cm ². The surviving fraction was determined by MTT assay. The assays were run in triplicate. Values of EC₅₀ (±SD) and LD₅₀ (±SD) in (a) and (c) were determined by a sigmoidal does–response (variable slope) analysis. Error bars are ± SD.

Inline graphic — Dark ( ) and phototoxicity ( ) of selenorhodamine thioamide 5 (a) toward HUT-78 cells with 0.0 and 1.0 J cm⁻² of laser light and varying concentrations of 5 and (b) with 5 × 10⁻⁸ M 5 ( ) and varying light dose (0.0, 0.5, 1.0, and 2.0 J cm⁻²) delivered at 630 ± 2 nm and a fluence rate of ~3.2 mW cm⁻². (c) Dark ( ) and phototoxicity ( ) of selenorhodamine amide 6 toward HUT-78 cells with 0.0 and 1.0 J cm⁻² of laser light and varying concentrations of 6 and (d) with 5 × 10⁻⁸ M 6 ( ) and varying light dose (0.0, 0.5, 1.0, and 2.0 J cm⁻²) delivered at 630 ± 2 nm and a fluence rate of ∼ 3.2 mW cm ². The surviving fraction was determined by MTT assay. The assays were run in triplicate. Values of EC₅₀ (±SD) and LD₅₀ (±SD) in (a) and (c) were determined by a sigmoidal does–response (variable slope) analysis. Error bars are ± SD.