Fig 4. ARF and INK4A are overexpressed in OPSCCs with an intact E2.

ARF (A) and INK4A (B) expression with HPV16 E2 gene disruption. Expression is defined as Y = 2maxΔCT- ΔCT where maxΔCT is the lower limit threshold of detection which equals 20 or 1. ΔCT is the experimental value minus the endogenous control (GAPDH). Statistical significance for difference in HPVE6 and E7 expression by site and between samples with an intact or disrupted HPV16 E2 gene was assessed by Mann-Whitney test.