Table 2. Adjusted spectral counts of the expression of the bacillithiol transferases and bacillithiol-related proteins during growth and sporulation.
Adjusted spectral countsa | ||||
---|---|---|---|---|
B. subtilis protein | Exponential phase | Stationary phase | t0 | t3 |
BstA | NDb | NDb | NDb | NDb |
BstB | 1.5 | 3.6 | 10.4 | 30.2 |
BstC | NDb | NDb | ND | NDb |
BstD | 3.8 | ND | 7.1 | 4.0 |
BstE | NDb | NDb | NDb | NDb |
BstF | NDb | NDb | NDb | NDb |
BstG | NDb | NDb | NDb | NDb |
BstH | NDb | NDb | NDb | NDb |
BrxA | 4.5 | 2.0 | 5.1 | 8.1 |
YtxJ | 1.1 | 12.0 | 14.9 | 34.0 |
CotEc | NDb | NDb | NDb | 2.8 |
SpoIIQc | NDb | NDb | NDb | 10.6 |
LC-MS/MS analysis using a TripleTOF 5600 (ABSCIEX) was performed on samples collected during exponential phase (OD600 = 0.4), stationary phase (OD600 = 4.0), at the initiation of sporulation (t0), and 3h after the induction of sporulation (t3). Adjusted spectral counts were calculated by counting the number of peptides with at least 95% confidence that were identified divided by the total amino acid length of the protein.
aTotal spectral counts for all proteins sum to 1; spectral counts for proteins were multiplied by 104.
bND: not detected.
cSporulation-specific proteins.