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. 2018 Feb 16;13(2):e0192977. doi: 10.1371/journal.pone.0192977

Table 2. Adjusted spectral counts of the expression of the bacillithiol transferases and bacillithiol-related proteins during growth and sporulation.

Adjusted spectral countsa
B. subtilis protein Exponential phase Stationary phase t0 t3
BstA NDb NDb NDb NDb
BstB 1.5 3.6 10.4 30.2
BstC NDb NDb ND NDb
BstD 3.8 ND 7.1 4.0
BstE NDb NDb NDb NDb
BstF NDb NDb NDb NDb
BstG NDb NDb NDb NDb
BstH NDb NDb NDb NDb
BrxA 4.5 2.0 5.1 8.1
YtxJ 1.1 12.0 14.9 34.0
CotEc NDb NDb NDb 2.8
SpoIIQc NDb NDb NDb 10.6

LC-MS/MS analysis using a TripleTOF 5600 (ABSCIEX) was performed on samples collected during exponential phase (OD600 = 0.4), stationary phase (OD600 = 4.0), at the initiation of sporulation (t0), and 3h after the induction of sporulation (t3). Adjusted spectral counts were calculated by counting the number of peptides with at least 95% confidence that were identified divided by the total amino acid length of the protein.

aTotal spectral counts for all proteins sum to 1; spectral counts for proteins were multiplied by 104.

bND: not detected.

cSporulation-specific proteins.